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首頁 > Cell line > B

B16-F10 Cell Line

 

Origin and General Characteristics

Cell Name

B16-F10 Cell Line

Organism

Mus musculus, Mouse

Tissue

Skin

Morphology

Mixture of spindle-shaped and epithelial-like cells

Growth Properties

Adherent

Biosafety Level

1

Culture Conditions and Handling

Complete Growth Medium

DMEM +10% FBS +1% P/S

Subculturing

Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Subcultivation Ratio

1:2-1:4

Medium Renewal

Every 2 to 3 days

Cryopreservation

Freeze medium: 60% Basal medium+30% FBS+10% DMSO
Storage temperature: Liquid nitrogen vapor phase

Culture Conditions

Atmosphere: Air, 95%; CO2, 5%
Temperature: 37℃

Special Features of the Cell Line

Applications

Transfection host.

 


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