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首頁 > Cell line > B

B95-8 Cell Line

 

Origin and General Characteristics

Cell Name

B95-8 Cell Line

Organism

Marmoset

Tissue

Blood

Morphology

Lymphoblast

Growth Properties

Suspension

Biosafety Level

2

Culture Conditions and Handling

Complete Growth Medium

RPMI-1640 +10% FBS +1% P/S

Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.

Subcultivation Ratio

1:2-1:4

Medium Renewal

Every 2 to 3 days

Cryopreservation

Freeze medium: 60% Basal medium+30% FBS+10% DMSO
Storage temperature: Liquid nitrogen vapor phase

Culture Conditions

Atmosphere: Air, 95%; CO2, 5%
Temperature: 37℃

 


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