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首頁 > Cell line > B

BALB/3T3 clone A31 Cell Line

 

Origin and General Characteristics

Cell Name

BALB/3T3 clone A31 Cell Line

Organism

Mus musculus, Mouse

Age

Embryo; 14 to 17 days gestation

Tissue

Embryo

Morphology

Fibroblast

Growth Properties

Adherent

Descriptions

The cells are extremely sensitive to contact inhibition of cell division, grow at a high dilution, exhibit a low saturation density and are highly susceptible to transformation in tissue culture by the oncogenic DNA virus, SV40, and murine sarcoma virusTested and found negative for ectromelia virus(mousepox).

Biosafety Level

2

Culture Conditions and Handling

Complete Growth Medium

DMEM +10% FBS +1% P/S

Subculturing

Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Subcultivation Ratio

1:2-1:4

Medium Renewal

Every 2 to 3 days

Cryopreservation

Freeze medium: 60% Basal medium+30% FBS+10% DMSO
Storage temperature: Liquid nitrogen vapor phase

Culture Conditions

Atmosphere: Air, 95%; CO2, 5%
Temperature: 37℃

Special Features of the Cell Line

Tumorigenic

No, the cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.

Effects

No, in immunosuppressed mice; Yes, in semisolid medium.

 


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