They secrete Insulin in response to glucose.The mouse carried the pseudogene construct composed of the SV40 early region controlled by the rat Insulin Ⅱgene promotor.The cells contain abundant Insulin and small amounts of glucagon and somatostatin. They secrete Insulin in response to glucose.
Culture Conditions and Handling
Complete Growth Medium
DMEM +15% heat-inactivated FBS +1% P/S
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.